Monday, 19 June 2023
| Opening | |
| From 14:00 | Registration |
| 17:00-17:30 | Workshop opening Pavel Hozak (Prague, Czech Republic) Chair of the Local Organizing Committee, Co-chairs of the workshop Yegor Vassetzky (Villejuif, France), Roman Lesyk (Lviv, Ukraine) |
| 17:30-17:40 | Wilhelm Bernhard Medal Ceremony |
| 17:40-18:20 | Wilhelm Bernhard Medal Lecture: From nucleolar morphology to phase separation in the cell nucleus Pavel Hozak, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| From 18:30 | Welcome Banquet |
Tuesday, 20 June 2023
| Workshop I: Nuclear Compartments and Gene Expression | |
| 09:00-10:20 | Session Chairs: Christian Schöfer & Marco Biggiogera |
| 09:00-09:20 | The Essence of Existence: from the Finalization of the 3D Genome Organization to a Novel Generalized/Unified Evolution Theory Tobias Aurelius Knoch, Biophysical Genomics, Mannheim, Germany |
| 09:20-09:40 | Intrinsically Disordered RGG/RG Repeats within Drosophila Nucleolar Proteins Bind Non-coding RNAs Expressed Upon Heat Shock for In Vivo Liquid Phase Condensation Patrick DiMario, Louisiana State University, Baton Rouge, USA |
| 09:40-10:00 | Most of the human pol II promoters have small regions with a distinctive physical property Ohyama Takashi, Waseda University, Tokyo, Japan |
| 10:00-10:20 | Epigenetic regulation and its modulation via the C-terminal tail of H2A.Z Gábor Szabó, University of Debrecen, Hungary |
| 10:20-10:50 | Coffee Break |
| 10:50-12:10 | Session Chairs: Reet Kurg & Masahiko Harata |
| 10:50-11:10 | HIRA-mediated loading of histone variant H3.3 regulates androgen-induced transcription in prostate cancer by retention of AR/BRD4 complex at enhancers Alexander M. Ishov, University of Florida College of Medicine, Gainesville, Florida, USA |
| 11:10-11:30 | Exploring the nuclear functions and nuclear presence of actin via in vivo methods Péter Borkúti, Biological Research Centre, Szeged, Hungary |
| 11:30-11:50 | Quantitative super-resolution microscopy reveals the phospholipid identity of the gene expression compartments in human cells and tissues Péter Hoboth, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| 11:50-12:10 | Differential Genomic Distribution of Human Histone H1 Variants Albert Jordan, Institute of Molecular Biology of Barcelona (IBMB-CSIC), Spain |
| 12:10-13:10 | Lunch break |
| 13:10-14:45 | Session Chairs: Reet Kurg & Masahiko Harata |
| 13:10-13:45 INVITED | Molecular principles of Cajal body formation David Staněk, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| 13:45-14:05 | Nuclear actin regulates the 3D genome organization Piergiorgio Percipalle, New York University Abu Dhabi (NYUAD), United Arab Emirates |
| 14:05-14:25 | The nuclear import of the drosophila moesin protein is regulated by cytoplasmic retention Zoltán Kovács, Biological Research Centre, Szeged, Hungary |
| 14:25-14:45 | Topological arrangement of poised rRNA genes and the nucleolar response to anti-cancer drug CX-5461 Christian Schöfer, Medical University of Vienna, Vienna Austria |
| 14:45-15:15 | Coffee Break |
| 15:15 – 16:50 | Session Chairs: Andrea Scharf & Yegor Vassetzky |
| 15:15-15:35 | Mercury Chloride reshapes heterochromatin domains and increases nucleolar activity in mouse hepatocytes Lorena Zannino, University of Pavia, Italy |
| 15:35-15:55 | Hyperinsulinemia in Brown Adipocytes: Both Insulin and Phosphorylated Insulin Receptor Enter the Nucleus and Reside in Heterochromatin Region Sara Stojanovic, University of Belgrade, Belgrade, Serbia |
| 15:55-16:15 | The intrinsically disordered N-terminus of cancer-testis antigen MAGEA10 is responsible for its expression, nuclear localization and aberrant migration Reet Kurg, Institute of Technology, University of Tartu, Estonia |
| 16:15-16:35 | Variability of Human rDNA and Transcription Activity of Ribosomal Genes Evgeny Smirnov, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czech Republic |
| 16:35-16:50 | A various response to different modes of anti-cancer treatment Oleg Demidov, INSERM UMR 1231, University of Burgundy, France |
| From 19:00 | Historical Center City Tour (signed up only) |
Wednesday, 21 June 2023
Workshop II: Nuclear Lipids and Phase Separation in Health and Disease | |
| (organized as a workshop of the COST Action 19105 – EpiLipidNET) | |
| 09:00-10:15 | Session Chairs: David Staněk & Martin Sztacho |
| 09:00-09:20 | The role of phosphatidylinositol 4,5-bisphosphate in transcription Ludovica Antiga, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| 09:20-09:55 INVITED | Phosphoinositide 3-kinase signalling in the nucleolus Aurélia E. Lewis, University of Bergen, Bergen, Norway |
| 09:55-10:15 | Mechanism of phase separation at PML nuclear bodies Peter Hemmerich, Leibniz-Institute on Aging – Fritz Lipman Institute, Germany |
| 10:15-10:45 | Coffee Break |
| 10:45-12:00 | Session Chairs: David Staněk & Martin Sztacho |
| 10:45-11:20 INVITED | HP1 mediated phase separation regulates gene expression in development and disease Heinrich Leonhardt, Ludwig Maximilians University Munich, Munich, Germany |
| 11:20-11:40 | Localization and molecular mechanisms of nuclear functions of phosphoinositide-3,4-bisphosphate Ana Miladinovic, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| 11:40-12:00 | Biomolecular condensates as a storage site for immature transcripts in plant cells Karolina Majewska, Nicolaus Copernicus University in Toruń, Poland |
| 12:00-13:00 | Lunch break |
| 13:00-14:15 | POSTER SESSION |
| 14:15-14:45 | Coffee break |
| 14:45 – 16:35 | Session Chairs: Peter Hemmerich & Pavel Hozak |
| 14:45-15:20 INVITED | Nuclear Inositide Signalling: Role of PLCs in Health and Disease Lucio Cocco, University of Bologna, Bologna, Italy |
| 15:20-15:40 | Molecular basis for interaction of intrinsically disordered proteins with nuclear PIP2 compartments Martin Sztacho, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| 15:40-16:15 INVITED | DNA methylation, methyl readers and heterochromatin organization Cristina Cardoso, Technical University of Darmstadt, Germany |
| 18:00 | Arranged bus departure from “IKEM” stop |
| From 19:00 | Social Dinner at the Strahov Monastery Brewery |
| 22:30 | Arranged bus departure from Strahov Monastery Brewery to EMMY Rezidence**** |
Thursday, 22 June 2023
| Workshop III: DNA Replication, Repair and Disease | |
| 09:00-10:20 | Session Chairs: Nikolajs Sjakste & Piotr Widlak |
| 09:00-09:20 | Repair and misrepair of telomeric DNA in dynamic interactions with PML bodies and nuclear lamina in doxorubicin-treated cancer cell lines Jekaterina Erenpreisa, Latvian Biomedicine Research & Study Centre, Riga, Latvia |
| 09:20-09:25 | Young Researcher Award Ceremony |
| 09:25-10:00 INVITED | ADP-ribose metabolism, DNA strand breaks and disease Hana Hanzlíková, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| 10:00-10:20 | Identification of potential readers of ADP-ribosylated DNA adducts in cell-free extracts Alexander Ishchenko, UMR9019 CNRS, Paris Saclay, Gustave Roussy, 94805 Villejuif, France |
| 10:20-10:50 | Coffee Break |
| 10:50-12:05 | Session Chairs: Nikolajs Sjakste & Piotr Widlak |
| 10:50-11:25 INVITED | PML compartment and nucleolus: when both structures meet together Pavla Vašicová, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| 11:25-11:45 | CHD5 promotes homologous recombination-specific chromatin changes to govern DNA double-strand break repair pathway choice David Yu, Emory University School of Medicine, Atlanta, Georgia, USA |
| 11:45-12:05 | Radiation-Induced Bystander Effect Mediated by Exosomes Involves the Replication Stress in Recipient Cells Piotr Widlak, Clinical Research Support Centre, Medical University of Gdańsk, Gdańsk, Poland |
| 12:05-13:05 | Lunch break |
| 13:05-14:40 | Session Chairs: Nikolajs Sjakste & Piotr Widlak |
| 13:05-13:40 | Detecting DNA Breaks, DNA Fragments and Cytosolic DNA in Cells In Vitro and Tissue Cryosections Using STRIDE Techniques Jurek Dobrucki, Jagiellonian University, Kraków, Poland |
| 13:40-14:00 | Why chromosmal translocations are cell linage-specific? Yegor Vassetzky, CNRS UMR9018, Institut Gustave Roussy, Université Paris Saclay, Villejuif, France |
| 14:00-14:20 | In-vivo approaches to study DNA repair in plant roots and protoplasts: rebooting laser microirradiation for plant samples Michal Franek, Central European Institute of Technology (CEITEC), Czech Republic |
| 14:20-14:40 | Protein crowding in DNA repair foci probed by fluorescence lifetime imaging microscopy Svitlana Levchenko, Jagiellonian University, Kraków, Poland |
| 14:40-15:10 | Coffee Break |
| Workshop IV: Novel Methods in Nuclear Research and Diagnostics | |
| 15:10 – 16:10 | Session Chairs: Anna von Mikecz & Gábor Szabó |
| 15:10-15:30 | Pollutants – single worm proteomics bring the environment to the lab Anette Limke, IUF – Leibniz Research Institute of Environmental Medicine at Heinrich-Heine-University Düsseldorf, Germany |
| 15:30-15:50 | Advanced correlative workflows for cryo-electron tomography on frozen-hydrated lamella for the study of multicellular organisms Vlada Filimonenko, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| 15:50-16:10 | The nucleus is the command center for populations and ecosystems Andrea Scharf, Missouri University of Science and Technology, USA |
| Free evening | |
Friday, 23 June 2023
Workshop V: Pathogenesis and Cancer | |
| (organized as a Symposium of the Society for Histochemistry) | |
| 09:00-10:30 | Session Chairs: Klara Weipoltshammer & Katarzyna Lisowska |
| 09:00-09:10 | Welcome from the SfH president Klara Weipoltshammer, Medical University of Vienna, Austria |
| 09:10-09:30 | Fat induced Nuclear Rupture Dennis Discher, University of Pennsylvania, Philadelphia, USA |
| 09:30-09:50 | Effects of Heat and Cold Shock on Epigenetics: Insights on Methylation and Chromatin Structure Claudio Casali, Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Italy |
| 09:50-10:10 | The interaction of two viral nuclear transcription factors HIV-1 Tat and EBV Zta induces immune evasion of EBV-infected B cells Anna Shmakova, Institut Gustave Roussy, CNRS UMR 9018 METSY, Villejuif, France |
| 10:10-10:30 | Targeting the 3D genomic and epigenetic changes in Mantle Cell Lymphoma Ana Kapurkhina, UMR 9018, CNRS, Université Paris Saclay, Institut Gustave Roussy, F-94805 Villejuif, France |
| 10:30-11:00 | Coffee Break |
| 11:00-12:20 | Session Chair: Nick Barlev |
| 11:00-11:20 | Gene expression profiles of GABAergic neuronal loss by nanoparticle exposure Dang Tri Le, IUF – Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany |
| 11:20-11:40 | Negative prognostic signature in ovarian cancer Katarzyna Lisowska, Center of Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, Gliwice, Poland |
| 11:40-12:00 | Transcriptional regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in mice Tomáš Venit, New York University Abu Dhabi (NYUAD), United Arab Emirates |
| 12:00-12:20 | Targeting mitochondria as a novel therapeutic approach to overcome TKI resistance of NSCLC cells: the role of paraptosis Nick Barlev, School of Medicine Nazarbayev University, Astana, Kazakhstan |
| 12:20-12:30 | Closing remarks |
| 12:30-14:00 | Lunch |
| From 14:00 | Departures |
Board Meetings
Seminary room (2.102) – 2nd floor
| MONDAY 19 JUNE 2023 15:30 – 16:30 I. WBW International Committee Meeting [MEMBERS ONLY] |
| THURSDAY 22 JUNE 2023 16:30 – 17:30 Society for Histochemistry Business Meeting |
| FRIDAY 23 JUNE 2023 12:30 – 13:30 II. WBW International Committee Meeting [MEMBERS ONLY] |
Social Program
More about social program
| MONDAY 19 JUNE 2023 From 18:30 Welcome Banquet |
| TUESDAY 20 JUNE 2023 From 19:00 Historical Centre City Tour (signed up participants) Meeting point: in front of the Powder Gate (in Czech “Prašná brána”), Nám. Republiky 5, 110 00 Staré Město |
| WEDNESDAY 21 JUNE 2023 From 19:00 Social Dinner at the Strahov Monastery Brewery, Strahovské nádvoří 301, 118 00 Praha 1-Hradčany 18:00 Bus departure from the IKEM bus stop, K Zelené louce 2a, 148 00 Praha-Kunratice 22:30 Bus departure from Strahov Monastery Brewery to EMMY Rezidence**** |