Monday, 19 June 2023
From 14:00Registration
17:00-17:30Workshop opening
Pavel Hozak (Prague, Czech Republic) Chair of the Local Organizing Committee,
Co-chairs of the workshop Yegor Vassetzky (Villejuif, France), Roman Lesyk (Lviv, Ukraine)
17:30-17:40Wilhelm Bernhard Medal Ceremony
17:40-18:20Wilhelm Bernhard Medal Lecture:
From nucleolar morphology to phase separation in the cell nucleus
Pavel Hozak, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
From 18:30Welcome Banquet
Tuesday, 20 June 2023
Workshop I: Nuclear Compartments and Gene Expression
09:00-10:20Session Chairs: Christian Schöfer & Marco Biggiogera
09:00-09:20The Essence of Existence: from the Finalization of the 3D Genome Organization to a Novel Generalized/Unified Evolution Theory
Tobias Aurelius Knoch, Biophysical Genomics, Mannheim, Germany
09:20-09:40Intrinsically Disordered RGG/RG Repeats within Drosophila Nucleolar Proteins Bind Non-coding RNAs Expressed Upon Heat Shock for In Vivo Liquid Phase Condensation
Patrick DiMario, Louisiana State University, Baton Rouge, USA
09:40-10:00Most of the human pol II promoters have small regions with a distinctive physical property
Ohyama Takashi, Waseda University, Tokyo, Japan
10:00-10:20Epigenetic regulation and its modulation via the C-terminal tail of H2A.Z
Gábor Szabó, University of Debrecen, Hungary
10:20-10:50Coffee Break
10:50-12:10Session Chairs: Reet Kurg & Masahiko Harata
10:50-11:10HIRA-mediated loading of histone variant H3.3 regulates androgen-induced transcription in prostate cancer by retention of AR/BRD4 complex at enhancers
Alexander M. Ishov, University of Florida College of Medicine, Gainesville, Florida, USA
11:10-11:30Exploring the nuclear functions and nuclear presence of actin via in vivo methods
Péter Borkúti, Biological Research Centre, Szeged, Hungary
11:30-11:50Quantitative super-resolution microscopy reveals the phospholipid identity of the gene expression compartments in human cells and tissues
Péter Hoboth, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
11:50-12:10Differential Genomic Distribution of Human Histone H1 Variants
Albert Jordan, Institute of Molecular Biology of Barcelona (IBMB-CSIC), Spain
12:10-13:10Lunch break
13:10-14:45Session Chairs: Reet Kurg & Masahiko Harata
Molecular principles of Cajal body formation
David Staněk, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
13:45-14:05Nuclear actin regulates the 3D genome organization
Piergiorgio Percipalle, New York University Abu Dhabi (NYUAD), United Arab Emirates
14:05-14:25The nuclear import of the drosophila moesin protein is regulated by cytoplasmic retention
Zoltán Kovács, Biological Research Centre, Szeged, Hungary
14:25-14:45Topological arrangement of poised rRNA genes and the nucleolar response to anti-cancer drug CX-5461
Christian Schöfer, Medical University of Vienna, Vienna Austria
14:45-15:15Coffee Break
15:15 – 16:50Session Chairs: Andrea Scharf & Yegor Vassetzky
15:15-15:35Mercury Chloride reshapes heterochromatin domains and increases nucleolar activity in mouse hepatocytes
Lorena Zannino, University of Pavia, Italy
15:35-15:55Hyperinsulinemia in Brown Adipocytes: Both Insulin and Phosphorylated Insulin Receptor Enter the Nucleus and Reside in Heterochromatin Region
Sara Stojanovic, University of Belgrade, Belgrade, Serbia
15:55-16:15The intrinsically disordered N-terminus of cancer-testis antigen MAGEA10 is responsible for its expression, nuclear localization and aberrant migration
Reet Kurg, Institute of Technology, University of Tartu, Estonia
16:15-16:35Variability of Human rDNA and Transcription Activity of Ribosomal Genes
Evgeny Smirnov, Institute of Biology and Medical Genetics, First Faculty of
Medicine, Charles University, Prague, Czech Republic
16:35-16:50A various response to different modes of anti-cancer treatment
Oleg Demidov, INSERM UMR 1231, University of Burgundy, France
From 19:00Historical Center City Tour (signed up only)
Wednesday, 21 June 2023
Workshop II: Nuclear Lipids and Phase Separation in Health and Disease
(organized as a workshop of the COST Action 19105 – EpiLipidNET)
09:00-10:15Session Chairs: David Staněk & Martin Sztacho
09:00-09:20The role of phosphatidylinositol 4,5-bisphosphate in transcription
Ludovica Antiga, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
Phosphoinositide 3-kinase signalling in the nucleolus
Aurélia E. Lewis, University of Bergen, Bergen, Norway
09:55-10:15Mechanism of phase separation at PML nuclear bodies
Peter Hemmerich, Leibniz-Institute on Aging – Fritz Lipman Institute, Germany
10:15-10:45Coffee Break
10:45-12:00Session Chairs: David Staněk & Martin Sztacho
HP1 mediated phase separation regulates gene expression in development and disease
Heinrich Leonhardt, Ludwig Maximilians University Munich, Munich, Germany
11:20-11:40Localization and molecular mechanisms of nuclear functions of phosphoinositide-3,4-bisphosphate
Ana Miladinovic, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
11:40-12:00Biomolecular condensates as a storage site for immature transcripts in plant cells
Karolina Majewska, Nicolaus Copernicus University in Toruń, Poland
12:00-13:00Lunch break
14:15-14:45Coffee break
14:45 – 16:35Session Chairs: Peter Hemmerich & Pavel Hozak
Nuclear Inositide Signalling: Role of PLCs in Health and Disease
Lucio Cocco, University of Bologna, Bologna, Italy
15:20-15:40Molecular basis for interaction of intrinsically disordered proteins with nuclear PIP2 compartments
Martin Sztacho, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
DNA methylation, methyl readers and heterochromatin organization
Cristina Cardoso, Technical University of Darmstadt, Germany
18:00Arranged bus departure from “IKEM” stop
From 19:00Social Dinner at the Strahov Monastery Brewery
22:30Arranged bus departure from Strahov Monastery Brewery to EMMY Rezidence****
Thursday, 22 June 2023
Workshop III: DNA Replication, Repair and Disease
09:00-10:20Session Chairs: Nikolajs Sjakste & Piotr Widlak
09:00-09:20Repair and misrepair of telomeric DNA in dynamic interactions with PML bodies and nuclear lamina in doxorubicin-treated cancer cell lines
Jekaterina Erenpreisa, Latvian Biomedicine Research & Study Centre, Riga, Latvia
09:20-09:25Young Researcher Award Ceremony
ADP-ribose metabolism, DNA strand breaks and disease
Hana Hanzlíková, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
10:00-10:20Identification of potential readers of ADP-ribosylated DNA adducts in cell-free extracts
Alexander Ishchenko, UMR9019 CNRS, Paris Saclay, Gustave Roussy, 94805 Villejuif, France
10:20-10:50Coffee Break
10:50-12:05Session Chairs: Nikolajs Sjakste & Piotr Widlak
PML compartment and nucleolus: when both structures meet together
Pavla Vašicová, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
11:25-11:45CHD5 promotes homologous recombination-specific chromatin changes to govern DNA double-strand break repair pathway choice
David Yu, Emory University School of Medicine, Atlanta, Georgia, USA
11:45-12:05Radiation-Induced Bystander Effect Mediated by Exosomes Involves the Replication Stress in Recipient Cells
Piotr Widlak, Clinical Research Support Centre, Medical University of Gdańsk, Gdańsk, Poland
12:05-13:05Lunch break
13:05-14:40Session Chairs: Nikolajs Sjakste & Piotr Widlak
13:05-13:40Detecting DNA Breaks, DNA Fragments and Cytosolic DNA in Cells In Vitro and Tissue Cryosections Using STRIDE Techniques
Jurek Dobrucki, Jagiellonian University, Kraków, Poland
13:40-14:00Why chromosmal translocations are cell linage-specific?
Yegor Vassetzky, CNRS UMR9018, Institut Gustave Roussy, Université Paris Saclay, Villejuif, France
14:00-14:20In-vivo approaches to study DNA repair in plant roots and protoplasts: rebooting laser microirradiation for plant samples
Michal Franek, Central European Institute of Technology (CEITEC), Czech Republic
14:20-14:40Protein crowding in DNA repair foci probed by fluorescence lifetime imaging microscopy
Svitlana Levchenko, Jagiellonian University, Kraków, Poland
14:40-15:10Coffee Break
Workshop IV: Novel Methods in Nuclear Research and Diagnostics
15:10 – 16:10Session Chairs: Anna von Mikecz & Gábor Szabó
15:10-15:30Pollutants – single worm proteomics bring the environment to the lab
Anette Limke, IUF – Leibniz Research Institute of Environmental Medicine at Heinrich-Heine-University Düsseldorf, Germany
15:30-15:50Advanced correlative workflows for cryo-electron tomography on frozen-hydrated lamella for the study of multicellular organisms
Vlada Filimonenko, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
15:50-16:10The nucleus is the command center for populations and ecosystems
Andrea Scharf, Missouri University of Science and Technology, USA
Free evening
Friday, 23 June 2023
Workshop V: Pathogenesis and Cancer
(organized as a Symposium of the Society for Histochemistry)
09:00-10:30Session Chairs: Klara Weipoltshammer & Katarzyna Lisowska
09:00-09:10Welcome from the SfH president
Klara Weipoltshammer, Medical University of Vienna, Austria
09:10-09:30Fat induced Nuclear Rupture
Dennis Discher, University of Pennsylvania, Philadelphia, USA
09:30-09:50Effects of Heat and Cold Shock on Epigenetics: Insights on Methylation and Chromatin Structure
Claudio Casali, Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Italy
09:50-10:10The interaction of two viral nuclear transcription factors HIV-1 Tat and EBV Zta induces immune evasion of EBV-infected B cells
Anna Shmakova, Institut Gustave Roussy, CNRS UMR 9018 METSY, Villejuif, France
10:10-10:30Targeting the 3D genomic and epigenetic changes in Mantle Cell Lymphoma
Ana Kapurkhina, UMR 9018, CNRS, Université Paris Saclay, Institut Gustave Roussy, F-94805 Villejuif, France
10:30-11:00Coffee Break
11:00-12:20Session Chair: Nick Barlev
11:00-11:20Gene expression profiles of GABAergic neuronal loss by nanoparticle exposure
Dang Tri Le, IUF – Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany
11:20-11:40Negative prognostic signature in ovarian cancer
Katarzyna Lisowska, Center of Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, Gliwice, Poland
11:40-12:00Transcriptional regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in mice
Tomáš Venit, New York University Abu Dhabi (NYUAD), United Arab Emirates
12:00-12:20Targeting mitochondria as a novel therapeutic approach to overcome TKI resistance of NSCLC cells: the role of paraptosis
Nick Barlev, School of Medicine Nazarbayev University, Astana, Kazakhstan
12:20-12:30Closing remarks
From 14:00Departures

Board Meetings

Seminary room (2.102) – 2nd floor

15:30 – 16:30 I. WBW International Committee Meeting [MEMBERS ONLY]
16:30 – 17:30 Society for Histochemistry Business Meeting
12:30 – 13:30 II. WBW International Committee Meeting [MEMBERS ONLY]

Social Program

More about social program

From 18:30 Welcome Banquet
From 19:00 Historical Centre City Tour (signed up participants)
Meeting point: in front of the Powder Gate (in Czech “Prašná brána”), Nám. Republiky 5, 110 00 Staré Město
From 19:00 Social Dinner at the Strahov Monastery Brewery, Strahovské nádvoří 301, 118 00 Praha 1-Hradčany
18:00 Bus departure from the IKEM bus stop, K Zelené louce 2a, 148 00 Praha-Kunratice
22:30 Bus departure from Strahov Monastery Brewery to EMMY Rezidence****