Scientific program

Monday, 19 June 2023
Opening
From 14:00	Registration
17:00-17:30	Workshop opening Pavel Hozak (Prague, Czech Republic) Chair of the Local Organizing Committee, Co-chairs of the workshop Yegor Vassetzky (Villejuif, France), Roman Lesyk (Lviv, Ukraine)
17:30-17:40	Wilhelm Bernhard Medal Ceremony
17:40-18:20	Wilhelm Bernhard Medal Lecture: From nucleolar morphology to phase separation in the cell nucleus Pavel Hozak, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
From 18:30	Welcome Banquet

Tuesday, 20 June 2023
Workshop I: Nuclear Compartments and Gene Expression
09:00-10:20	Session Chairs: Christian Schöfer & Marco Biggiogera
09:00-09:20	The Essence of Existence: from the Finalization of the 3D Genome Organization to a Novel Generalized/Unified Evolution Theory Tobias Aurelius Knoch, Biophysical Genomics, Mannheim, Germany
09:20-09:40	Intrinsically Disordered RGG/RG Repeats within Drosophila Nucleolar Proteins Bind Non-coding RNAs Expressed Upon Heat Shock for In Vivo Liquid Phase Condensation Patrick DiMario, Louisiana State University, Baton Rouge, USA
09:40-10:00	Most of the human pol II promoters have small regions with a distinctive physical property Ohyama Takashi, Waseda University, Tokyo, Japan
10:00-10:20	Epigenetic regulation and its modulation via the C-terminal tail of H2A.Z Gábor Szabó, University of Debrecen, Hungary
10:20-10:50	Coffee Break
10:50-12:10	Session Chairs: Reet Kurg & Masahiko Harata
10:50-11:10	HIRA-mediated loading of histone variant H3.3 regulates androgen-induced transcription in prostate cancer by retention of AR/BRD4 complex at enhancers Alexander M. Ishov, University of Florida College of Medicine, Gainesville, Florida, USA
11:10-11:30	Exploring the nuclear functions and nuclear presence of actin via in vivo methods Péter Borkúti, Biological Research Centre, Szeged, Hungary
11:30-11:50	Quantitative super-resolution microscopy reveals the phospholipid identity of the gene expression compartments in human cells and tissues Péter Hoboth, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
11:50-12:10	Differential Genomic Distribution of Human Histone H1 Variants Albert Jordan, Institute of Molecular Biology of Barcelona (IBMB-CSIC), Spain
12:10-13:10	Lunch break
13:10-14:45	Session Chairs: Reet Kurg & Masahiko Harata
13:10-13:45 INVITED	Molecular principles of Cajal body formation David Staněk, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
13:45-14:05	Nuclear actin regulates the 3D genome organization Piergiorgio Percipalle, New York University Abu Dhabi (NYUAD), United Arab Emirates
14:05-14:25	The nuclear import of the drosophila moesin protein is regulated by cytoplasmic retention Zoltán Kovács, Biological Research Centre, Szeged, Hungary
14:25-14:45	Topological arrangement of poised rRNA genes and the nucleolar response to anti-cancer drug CX-5461 Christian Schöfer, Medical University of Vienna, Vienna Austria
14:45-15:15	Coffee Break
15:15 – 16:50	Session Chairs: Andrea Scharf & Yegor Vassetzky
15:15-15:35	Mercury Chloride reshapes heterochromatin domains and increases nucleolar activity in mouse hepatocytes Lorena Zannino, University of Pavia, Italy
15:35-15:55	Hyperinsulinemia in Brown Adipocytes: Both Insulin and Phosphorylated Insulin Receptor Enter the Nucleus and Reside in Heterochromatin Region Sara Stojanovic, University of Belgrade, Belgrade, Serbia
15:55-16:15	The intrinsically disordered N-terminus of cancer-testis antigen MAGEA10 is responsible for its expression, nuclear localization and aberrant migration Reet Kurg, Institute of Technology, University of Tartu, Estonia
16:15-16:35	Variability of Human rDNA and Transcription Activity of Ribosomal Genes Evgeny Smirnov, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czech Republic
16:35-16:50	A various response to different modes of anti-cancer treatment Oleg Demidov, INSERM UMR 1231, University of Burgundy, France
From 19:00	Historical Center City Tour (signed up only)

Wednesday, 21 June 2023
Workshop II: Nuclear Lipids and Phase Separation in Health and Disease

(organized as a workshop of the COST Action 19105 – EpiLipidNET)
09:00-10:15	Session Chairs: David Staněk & Martin Sztacho
09:00-09:20	The role of phosphatidylinositol 4,5-bisphosphate in transcription Ludovica Antiga, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
09:20-09:55 INVITED	Phosphoinositide 3-kinase signalling in the nucleolus Aurélia E. Lewis, University of Bergen, Bergen, Norway
09:55-10:15	Mechanism of phase separation at PML nuclear bodies Peter Hemmerich, Leibniz-Institute on Aging – Fritz Lipman Institute, Germany
10:15-10:45	Coffee Break
10:45-12:00	Session Chairs: David Staněk & Martin Sztacho
10:45-11:20 INVITED	HP1 mediated phase separation regulates gene expression in development and disease Heinrich Leonhardt, Ludwig Maximilians University Munich, Munich, Germany
11:20-11:40	Localization and molecular mechanisms of nuclear functions of phosphoinositide-3,4-bisphosphate Ana Miladinovic, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
11:40-12:00	Biomolecular condensates as a storage site for immature transcripts in plant cells Karolina Majewska, Nicolaus Copernicus University in Toruń, Poland
12:00-13:00	Lunch break
13:00-14:15	POSTER SESSION
14:15-14:45	Coffee break
14:45 – 16:35	Session Chairs: Peter Hemmerich & Pavel Hozak
14:45-15:20 INVITED	Nuclear Inositide Signalling: Role of PLCs in Health and Disease Lucio Cocco, University of Bologna, Bologna, Italy
15:20-15:40	Molecular basis for interaction of intrinsically disordered proteins with nuclear PIP2 compartments Martin Sztacho, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
15:40-16:15 INVITED	DNA methylation, methyl readers and heterochromatin organization Cristina Cardoso, Technical University of Darmstadt, Germany
18:00	Arranged bus departure from Emmy Rezidence****
From 19:00	Social Dinner at the Strahov Monastery Brewery
22:30	Arranged bus departure from Strahov Monastery Brewery to EMMY Rezidence****

Thursday, 22 June 2023
Workshop III: DNA Replication, Repair and Disease
09:00-10:20	Session Chairs: Nikolajs Sjakste & Piotr Widlak
09:00-09:20	Repair and misrepair of telomeric DNA in dynamic interactions with PML bodies and nuclear lamina in doxorubicin-treated cancer cell lines Jekaterina Erenpreisa, Latvian Biomedicine Research & Study Centre, Riga, Latvia
09:20-09:25	Young Researcher Award Ceremony
09:25-10:00 INVITED	ADP-ribose metabolism, DNA strand breaks and disease Hana Hanzlíková, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
10:00-10:20	Identification of potential readers of ADP-ribosylated DNA adducts in cell-free extracts Alexander Ishchenko, UMR9019 CNRS, Paris Saclay, Gustave Roussy, 94805 Villejuif, France
10:20-10:50	Coffee Break
10:50-12:05	Session Chairs: Nikolajs Sjakste & Piotr Widlak
10:50-11:25 INVITED	PML compartment and nucleolus: when both structures meet together Pavla Vašicová, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
11:25-11:45	CHD5 promotes homologous recombination-specific chromatin changes to govern DNA double-strand break repair pathway choice David Yu, Emory University School of Medicine, Atlanta, Georgia, USA
11:45-12:05	Radiation-Induced Bystander Effect Mediated by Exosomes Involves the Replication Stress in Recipient Cells Piotr Widlak, Clinical Research Support Centre, Medical University of Gdańsk, Gdańsk, Poland
12:05-13:05	Lunch break
13:05-14:40	Session Chairs: Nikolajs Sjakste & Piotr Widlak
13:05-13:40 INVITED	The role of nuclear PI(4,5)P2 in papillomavirus associated cancer development Baki Akgül, Institute of Virology, University Hospital Cologne, Germany
13:40-14:00	Why chromosmal translocations are cell linage-specific? Yegor Vassetzky, CNRS UMR9018, Institut Gustave Roussy, Université Paris Saclay, Villejuif, France
14:00-14:20	In-vivo approaches to study DNA repair in plant roots and protoplasts: rebooting laser microirradiation for plant samples Michal Franek, Central European Institute of Technology (CEITEC), Czech Republic
14:20-14:40	Protein crowding in DNA repair foci probed by fluorescence lifetime imaging microscopy Svitlana Levchenko, Jagiellonian University, Kraków, Poland
14:40-15:10	Coffee Break
Workshop IV: Novel Methods in Nuclear Research and Diagnostics
15:10 – 16:30	Session Chairs: Anna von Mikecz & Gábor Szabó
15:10-15:30	*Detecting DNA Breaks, DNA Fragments and Cytosolic DNA in Cells In Vitro* and Tissue Cryosections Using STRIDE Techniques** Jurek Dobrucki, Jagiellonian University, Kraków, Poland
15:30-15:50	Pollutants – single worm proteomics bring the environment to the lab Anette Limke, IUF – Leibniz Research Institute of Environmental Medicine at Heinrich-Heine-University Düsseldorf, Germany
15:50-16:10	Advanced correlative workflows for cryo-electron tomography on frozen-hydrated lamella for the study of multicellular organisms Vlada Filimonenko, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic
16:10-16:30	The nucleus is the command center for populations and ecosystems Andrea Scharf, Missouri University of Science and Technology, USA
Free evening

Friday, 23 June 2023
Workshop V: Pathogenesis and Cancer
(organized as a Symposium of the Society for Histochemistry)
09:00-10:30	Session Chairs: Klara Weipoltshammer & Katarzyna Lisowska
09:00-09:10	Welcome from the SfH president Klara Weipoltshammer, Medical University of Vienna, Austria
09:10-09:30	Fat induced Nuclear Rupture Dennis Discher, University of Pennsylvania, Philadelphia, USA
09:30-09:50	Effects of Heat and Cold Shock on Epigenetics: Insights on Methylation and Chromatin Structure Claudio Casali, Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Italy
09:50-10:10	The interaction of two viral nuclear transcription factors HIV-1 Tat and EBV Zta induces immune evasion of EBV-infected B cells Anna Shmakova, Institut Gustave Roussy, CNRS UMR 9018 METSY, Villejuif, France
10:10-10:30	Targeting the 3D genomic and epigenetic changes in Mantle Cell Lymphoma Ana Kapurkhina, UMR 9018, CNRS, Université Paris Saclay, Institut Gustave Roussy, F-94805 Villejuif, France
10:30-11:00	Coffee Break
11:00-12:20	Session Chair: Nick Barlev
11:00-11:20	Gene expression profiles of GABAergic neuronal loss by nanoparticle exposure Dang Tri Le, IUF – Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany
11:20-11:40	Negative prognostic signature in ovarian cancer Katarzyna Lisowska, Center of Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, Gliwice, Poland
11:40-12:00	Transcriptional regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in mice Tomáš Venit, New York University Abu Dhabi (NYUAD), United Arab Emirates
12:00-12:20	Targeting mitochondria as a novel therapeutic approach to overcome TKI resistance of NSCLC cells: the role of paraptosis Nick Barlev, School of Medicine Nazarbayev University, Astana, Kazakhstan
12:20-12:30	Closing remarks
12:30-14:00	Lunch
From 14:00	Departures

Board Meetings

Social Program